How to use TB-500 (Thymosin beta 4) for healing purposes

HOW TO USE TB-500 Thymosin beta 4 for healing benefits

Research has shown that the best results are achieved when using sterile saline solution (Sodium Chloride Injection BP 0.9%) to mix TB-500 (thymosin beta4).

Only prepare the vials that will be used immediately. If using doses that are lower than the recommended dose, prepared vials must be stored in the fridge and be used within 8 days.

Vial preparation:
Remove the plastic protective cap of the sealed vial containing the powder of peptides.

Insert the filled syringe of 2ml of sodium chloride solution into the rubber top of the vial and release the 2ml of sodium chloride into the vial.

Remove the syringe and gently rotate the vial until the powder is completely dissolved.

Ensure that the solution is well mixed with all powder thoroughly dissolved for maximum safety and effectiveness.

A Subcutaneous (S/C) inj.

Research studies have further shown that one (2ml. vial) Sub-Q Inj. each week for six consecutive weeks provides the best results.  There after, use only one (2ml. vial) per month.  It’s best to give the Sub-Q Inj. 6 days before intense work outs. Therefore for best results, one vial per Sub-Q injection per week for 6 consecutive weeks, then 1 vial per month (the glass vial is 2ml, with 10mg TB-500 per vial), so it is 10mg/2ml).

No known contraindication with tb4 peptide products, however tb500 has not been thoroughly tested to know its full effects.
The product should not be used if the subject is pregnant or breast feeding.

TB500 Storage:
Store the product at room temperature in a non humid environment. An opened vile can be kept for up to 8 days in refrigerated storage.


What is TB-500?
TB-500 is a synthetic version of the naturally occurring peptide present in virtually all human and animal cells, Thymosin Beta 4 (TB-500). It is a first-in-class drug candidate that promotes the following*:

•  Endothelial (blood vessels) cell differentiation
•  Angiogenesis (growth of new blood cells from pre-existing vessels) in dermal tissues
•  Keratinocyte migration
•  Collagen deposition; and
•  Decreases inflammation.
One of TB500 key mechanisms of action is its ability to regulate the cell-building protein, Actin, a vital component of cell structure and movement. Of the thousands of proteins present in cells, actin represents up to 10% of the total proteins which therefore plays a major role in the genetic makeup of the cell.

This potent peptide is a member of a ubiquitous family of 16 related molecules with a high conservation of sequence and localization in most tissues and circulating cells in the body. Thymosin beta-4 not only binds to actin, but also blocks actin polymerization and is the actin-sequestering molecule.

Tß4 was identified as a gene that was up-regulated four-to-six fold during early blood vessel formation and found to promote the growth of new blood cells from the existing vessels. This peptide is present in wound fluid and when administered subcutaneously, it promotes wound healing, muscle building and speeds up recovery time of muscles fibres and their cells.

An additional key factor of TB500 is that it promotes cell migration through a specific interaction with actin in the cell cytoskeleton. It has been demonstrated that a central small amino acid long-actin binding domain has both blood cell reproduction and wound healing characteristics. These characteristics are uncovered by accelerating the migration of endothelial cells and keratinocytes. It also increases the production of extracellular matrix-degrading enzymes.

Research confirms that TB500 is a potent, naturally occurring wound repair factor with anti-inflammatory properties. TB-500 is different from other repair factors, such as growth factors, in that it promotes endothelial and keratinocyte migration. It also does not bind to the extracellular matrix and has a very low molecular weight meaning it can travel relatively long distances through tissues.

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