Tag Archive for PEGylated Mechano Growth Factor

peg-MGF + IGF1-LR3 bodybuilding peptide cycle

peg-MGF + IGF1-LR3

Mechano Growth Factor (MGF). We know things like IGF creates new muscle cells
and helps drop body fat and MGF helps facilitate this muscle growth and fat loss.
Most of us are familiar with the peptides Insulin-Like Growth Factor (IGF) and
In Mechano Growth Factor (MGF). We know things like IGF creates new muscle cells the beginning:

IGF first made its way onto the bodybuilding scene most people were injecting it every day and noticed that after somewhere around 30 days the effects of IGF wore off. This was blamed on receptor “down-regulation” or “desensitization”. To combat this people started a 5-on 2-off rotation and then went to a post workout only rotation which extended the time on IGF an additional 10-20 days. But the problem with the lack of “receptor response” was still present.

Then MGF came onto the scene. MGF was suppose to be the next best thing in bodybuilding. It was suppose to be more anabolic than AAS and better suited for building new muscle than IGF, the problem was that it wasn’t. Because of its instability it was quickly broken down once injected into the body, to prevent this PegMGF was created. This new MGF was now able to survive in the body from anywhere in the 1-3 days. Combining this new peptide discovery with IGF was suppose to be able to make all of us the next Mr. Olympia. But what happened? Why do we not see a flood of new pros?

What happened:

In theory these two peptides should cause some great results, the problem is they do not work together very well unless your timing is spot on (I will go into greater detail later). In short stem cells in the presence of MGF will cause the cells to split and multiply. When the cells are multiplying they cannot form new tissue and the effects of IGF are completely blocked. So the two together are not very compatible.

Then why not just use IGF? Well most do use IGF only and get great results, but there is that pesky business of receptor “down-regulation” or “desensitization” and you have to end a cycle of IGF after 30-50 days and there is no way to prevent it… or is there?

The science:

In a natural system (Our Body) we have peaks and dips with MGF and IGF levels. The reasons for these peaks and dips are to create the ideal amount of cells to repair and create new tissue. After strenuous exercise the levels of MGF in the body (more specifically in the muscle just trained) increase dramatically and there is a dramatic decrease of IGF levels. The reason for this is because MGF causes stem cells to proliferate (split and multiply). This process ensures that there are enough cells available to make repairs and to create new tissue in order for the tissue to function efficiently and properly (in this case skeletal muscle tissue). As mentioned above in the presence of MGF there is no need for IGF because it is rendered useless and cannot activate the stem cells, so this explains the bodies response to decrease IGF levels.

In 12-36 hours MGF levels begin to drop and there is a direct correlation in the rise of IGF levels within the body. Stem cells have proliferated and now the IGF will bind to the proper receptors and cause differentiation (force the stem cells to form into a specific cell for a specific tissue type). This process repeats every time you exercise and keeps the natural system in an efficient state.

When flooding the body with these artificial peptides a person will change this natural system dramatically. A person using MGF only is causing stem cell proliferation while at the same time preventing IGF to perform its duties of creating new tissue from those newly created stem cells. A person using IGF only is depleting the supply of stem cells at a rate much faster than the body can keep up, leading to a depletion of available stem cells and not receptor “down-regulation” or “desensitization.”

This sounds like a lose-lose situation. You are throwing off your body’s final tuned muscle repairing mechanism, depleting valuable stem cells or creating too many stem cells for your body to deal with. Why bother?

The climax:

The reason why we bother is because we want to reach or goals. We want to be the biggest bodybuilder, the best powerlifter or whatever it is that we are training so hard for. These peptides are a great addition to our arsenal, but learning to use them properly is the key to utilizing their benefits.

The key is retraining the way we think about MGF and IGF. We have to understand that we are dealing with the creation and depletion of stem cells that are going to be responsible for our muscular growth. Our bodies do not have a constant supply of these stem cells and our bodies will not naturally utilize all of the stem cells it has created. Since we are trying to artificially manipulate the amount and utilization of these stem cells we have to look at this is a different manner.

While we may never get the exogenous MGF and IGF levels just right so that we may counteract the depletion of the stem cells we can adjust our protocols in a way that will increase the amount of time a person can use and respond to both peptides.

The Conclusion Part 1, MGF:

We know that the PegMGF will stay in the body for several days and we know that while in the presence of MGF stem cells will proliferate and the use of IGF is futile. We also know that MGF without the peg is of little to no help because of how quickly exogenous MGF is broken down within the body. So what are the options?

Well both can be of use! PegMGF can be of great use as long as the individual using the peptide in conjuction with IGF understands that the two peptides must be injected in a manner that falls outside of the current way of thinking. And MGF without the Peg addition can also be utilized as long as you don’t mind being a pin cushion.

The key with MGF is to learn to either follow your bodies natural peaks and dips of MGF levels and force proliferation on a larger scale with MGF, or to force a longer period of cell proliferation with the use of PegMGF. The key is you have to have stem cells in order to create new muscle tissue.

The Conclusion Part 2, IGF:

Now that we have hit the MGF part of the cycle, now we move into the part of the cycle that utilizes the stem cells. Again we want to either follow the body’s natural peaks and dips of IGF levels or we are going to want to cause a prolonged forced differentiation phase. The latter of the two options is simply following standard protocol of everyday injections or 5-on 2-off. The other is all about timing. We know that MGF levels peak in the body after strenuous exercise, so why would you want to inject a substance that is useless in the presence of MGF right when MGF levels are at their highest? The answer is you don’t! You will want to wait and inject the IGF 24hrs after the exercise. This will give ample time for the MGF peak to start to dip and can closely mimic the natural rise in IGF levels. This will allow for a person to use a more efficient dose since the timing of the IGF will correspond closely to the dip in MGF levels resulting in greater utilization of the exogenous IGF.

The cycles:

There are a few cycles I would recommend.

The first being IGF only. It works, maybe not the most efficient plan out there but it does work none-the-less.

The second would be the PegMGF/IGF combination. This is not the most efficient methos but should significantly increase the amount of time one can be on an MGF/IGF cycle and still see positive results. (This may have to be altered according to your training schedule)

Sunday – Off Training – Mid-day PegMGF 200-300mcg
Monday – Training (Afternoon)
Tuesday – Off Training – Afternoon IGF 40-80mcg
Wednesday – Training (Afternoon)
Thursday – Off Training – Afternoon IGF 40-80mcg
Friday – Training (Afternoon)
Saturday – Off Training – Afternoon IGF Injection 40-80mcg

The addition of the PegMGF will cause an increase in amount and duration of stem cell proliferation and should subside about the period of the first IGF injection. While this will not keep stem cell levels stable it should prevent the drastic decrease in stem cell numbers seen with IGF only cycles and should significantly increase cycle length.

The third would be the use of regular MGF plus the addition of IGF and woulld closely mimic the natural system.

Sunday – Off Training
Monday – Training (afternoon) – 1hr PWO MGF 50mcg in muscles trained
Tuesday – Off Training – Afternoon IGF 40-80mcg
Wednesday – Training (Afternoon) – 1hr PWO MGF 50mcg in muscles trained
Thursday – Off Training – Afternoon IGF 40-80mcg
Friday – Training (Afternoon) – 1hr PWO MGF 50mcg in muscles trained
Saturday – Off Training – Afternoon IGF 40-80mcg

This protocol should closely mimic natural peaks and dips in MGF and IGF within the specific muscles being trained. While regular MGF is short lived in the body the addition on the regular MGF 1 hour post workout should cause an increase in cell proliferation beyond the natural system’s ability and should create a larger pool of stem cells for the utilization of IGF therapy.

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PEGylated Mechano Growth Factor (MGF)

PEGylated Mechano Growth Factor (MGF)




Quick summary: MGF is a splice variant of the IGF produced by a frame shift if the IGF gene. MGF increase the muscle stem cell count, so that more may fuse and become part of adult muscle cells. This is a process required for adult muscle cells to continue growing.

Why PEGylate MGF?
MGF exhibits local effects in skeletal muscle and without modification is not systemic (can’t travel through the body). The problem with synthetic MGF is that it is introduced IM and is water based so it goes into the blood stream. MGF is not stable in the blood stream for more than a matter of minutes. Biologically produced MGF is made locally and does not enter the bloodstream and is short acting so stability is not an issue. By PEGylating the MGF we can make synthetic MGF injected IM almost as efficient as local produced MGF. Clinically proven Advanced Pegylation, the technology of polyethylene glycol (PEG) conjugation, holds significant promise in maintaining effective plasma concentrations of systemically administered drugs. It does this by surrounding part of the peptide with a unique structure made of polyethylene glycol, which can be attached to a protein molecule. The result of a correct PEGylation is simlar to the protective mechanism of a turtle shell. The polyethylene glycol groups protect the peptide but don’t surround it completely. The active sites of the peptide are still free to do their biological function. In this case the shell is a negative charged shield against positively charged compounds that would affect the protein. This also provides a nice steric chamber for the peptide to reside in. So it’s a happy turtle

Neurological research has shown that utilizing PEGylated MGF resulted in a longer more stable acting version of the MGF peptide in serum/blood.

Bottom line
PEGylation can improve performance and dosing convenience of peptides, proteins, antibodies, oligonucleotides and many small molecules by optimizing pharmacokinetics, increasing bioavailability, and decreasing immunogenicity and dosing frequency. PEGylation also can increase therapeutic efficacy by enabling increased drug concentration, improved biodistribution, and longer dwell time at the site of action. As a result, therapeutic drug concentrations can be achieved with less frequent dosing—a significant benefit to patients who are taking injected drugs.

The PEG itself does not react in the body and is very safe. PEG has been approved by the US Food and Drug Administration (FDA) as a base or vehicle for use in foods and cosmetics and in injectable, topical, rectal and nasal pharmaceutical formulations. PEG has demonstrated little toxicity, is eliminated intact by the kidneys or in the feces and lacks immunogenicity. The risk associated with current PEGylated drugs are due to the way the drug itself acts not the PEG. MGF, as it is being currently sold, is getting a bad rep from people due to the fact they feel that they are not seeing gains from it. Many people believe that the use of MGF in their cycles or protocols just flat out won’t work, however, this is far from the truth.
More MGF information

Complete Overview of MGF or IGF-IEc

From its sequence, MGF is derived from the IGF-I gene by alternative splicing and has different 3′ exons to the liver or systemic type (IGF-IEa). It has a 49 base pair insert in the human, and a 52 base pair insert in rodents, within the E domain of exon 5. This insert results in a reading frame shift, with a different carboxy (C) terminal sequence to that of systemic IGF-IEa. MGF and the other IGF isoforms have the same 5′ exons that encode the IGF-I ligand-binding domain. Processing of pro-peptide yields a mature peptide that is involved in upregulating protein synthesis. However, there is evidence that the carboxy-terminal of the MGF peptide also acts as a separate growth factor. This stimulates division of mononucleated myoblasts or satellite (stem) cells, thereby increasing the number available for local repair

During the early stage of skeletal muscle development, myoblasts (muscle stem cells) fuse to form syncytial myotubes, which become innervated and develop into muscle fibres. Thereafter, mitotic proliferation of nuclei within the muscle fibres ceases. However, during postnatal (after development) growth, additional nuclei are provided by satellite cells (myoblast) fusing with myotubules. Muscle damage-recovery seems to have a similar cellular mechanism, in that satellite cells become activated and fuse with the damaged muscle fibres (reviewed by Goldring et al. 2002). This is also pertinent to certain diseases such as muscular dystrophy in which muscle tissue is not maintained and which have been associated with a deficiency in active satellite (stem) cells (Megeney et al. 1996; Seale & Rudnicki, 2000) and in myogenic factors (Heslop et al. 2000). Skeletal muscle mass and regenerative capacity have also been shown to decline with age (Sadeh, 1988; Carlson et al. 2001). The reduced capacity to regenerate in older muscle seems to be due to the decreased ability to activate satellite cell proliferation (Chakravarthy et al. 2000). The markedly lower expression of MGF in older rat muscles (Owino et al. 2001) and human muscle (Hameed et al. 2003) in response to mechanical overload has been associated with the failure to activate satellite cells, leading to age-related muscle loss (Owino et al. 2001). Your muscle cels can not grow once they have reached a certain size unless they obtain more nuclei from the myoblast. MGF increases the myblast available to donate their nuclei to the adult muscle cell.

“MGF appears to have a dual action in that, like the other IGF-I isoforms, it upregulates protein synthesis as well as activating satellite cells. However, the latter role of MGF is probably more important as most of the mature IGF-I will be derived from IGF-IEa during the second phase of repair. Nevertheless, it has been shown that MGF is a potent inducer of muscle hypertrophy in experiments in which the cDNA of MGF was inserted into a plasmid vector and introduced by intramuscular injection. This resulted in a 20 % increase in the weight of the injected muscle within 2 weeks, and the analyses showed that this was due to an increase in the size of the muscle fibres (Goldspink, 2001). Similar experiments by other groups have also been carried out using a viral construct containing the liver type of IGF-I, which resulted in a 25 % increase in muscle mass, but this took over 4 months to develop (Musaro et al. 2001). Hence, the dual role MGF plays in inducing satellite cell activation as well as protein synthesis suggests it is much more potent than the liver type or IGF-IEa for inducing rapid hypertrophy.”

These results are based on actual transplantation of the DNA coding for the peptides. This is a permanent effect and much more potent than IM injections of the peptide itself. You will not see a 20% increase in muscle mass through IM injections as claimed above.

PEGylated MGF dosing Protocols

The PEGylated version is going to be much longer lasting making a 1-2 dose per week procedure possible. I still think its best used with IGF or AAS to maximize the benefits so here are some sample protocols

Once a week PEG MGF/ IGF
Sunday 100-300 mcg MGF you can choose to site inject if you wish. I think splitting large doses may benefit.
Monday –Fri IGF 50mcg e/d

Twice a week PEG MGF / IGF
Sunday and Wed MGF 50-150 mcg
MT, ThF IGF 50 mcg

These protocols are just to start as this is brand new feel free to tweak them if you like. I will update them after we have done some testing.

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