Tag Archive for Reconstituting peptides

Peptide mixing Information

Peptide mixing Information

Peptides come in the form of lyophilized (freeze dried) powder. The amount of powder/product is stated in International Units (IU’s) or in Milligrams (MG).
Melanotan peptides (Melanotan 1 & Melanotan 2), PT-141 Bremelanotide, GH Fragment, Ipamorelin, CJC-1295 & GHRPs (GRHP-2 & GHRP-6), HGH, HCG, et cetera use Bacteriostatic Water (BW). Bacteriostatic Water for injection, USP is a sterile, nonpyrogenic preparation of water for injection containing 0.9% of benzyl alcohol added as a bacteriostatic preservative. It is supplied in a multiple-dose container from which repeated withdrawals may be made to dilute or dissolve drugs for injection. The pH is 5.7 (4.5 to 7.0)

For IGF use an acetic acid solution (.6%) which is 7 parts distilled water and 1 part vinegar to reconstitute. You must filter the distilled water and white wine vinegar through a sterile 20 micron syringe filter before use. Sodium Chloride (NaCl) is used to buffer the injection.

1.) Take an alcohol swab to the stopper of both your peptide vial and the vial of the diluent.

2.) Draw your preferred diluent (BW) with a 1cc syringe. Choose an amount that will make measuring the final product simple.

1ml(cc) per 10 mg vial of Melanotan would mean each 10 tick marks on a U100 slin syringe would equal 1mg of Melanotan
1ml(cc) per 10 IU vial of HGH would mean each 10 tick marks on a U100 slin syringe would equal 1 IU of HGH

3.) Take the syringe with the diluent and push it into the vial of lyophilized powder letting the diluent dissolve the peptide. Many (not all) peptides are sealed with vacuum pressure, be careful.

4.) After diluent has been added to the vial, gentling swirl the vial until the lyophilized powder has dissolved and you are left with a clear liquid. The peptide is now reconstructed, ready for measurement and usage.
5.) Store your now reconstituted research peptides in the refrigerator.
Peptide Measurement
After successfully reconstituting your peptide, measure the desired amount out for injection. Use a U100 insulin syringe to draw out and inject your product.

Since you know the amount of IU’s/MG’s in your vial, we divide this out as follows:
You will need to know the following to be successful: 1ml = 1cc = 100 IU’s

We take our dose from the label of the dry lyophilized powder and we divide that into the amount of diluent used.

Example- We used 1cc(ml) of water. We have a 10 IU vial of HGH.
From our formula above we know that 1cc = 100 IU’s, so we have 100 IU’s of water.
We now divide the 100 IU’s (the amount of our water) by 10 IU’s (the amount of our HGH)
100 IU / 10 IU = 10

This 10 will perfectly correspond with the markings on a U100 insulin syringe. In our example every 10 mark on our syringe will equal 1 IU of HGH. Want to draw out 2 IU’s of GH? ….draw out to the 20 mark on the syringe (1/5th of the syringe).

Say you have a 1mg vial and you add 1ML you get
1000mcg/1mL: 10 mcg per IU
1000mcg/2mL: 5 mcg per IU

Say you have a 10mg vial and you add 1ML you get
10mg/1mL: 1 mg per 10 IU
10mg/2mL: .5 mg per 10 IU

Say you have a 20mg vial and you add 1ML you get
20mg/1mL: 2 mg per 10 IU
20mg/2mL: 1 mg per 10 IU

Say you have a 10iu vial and you add 1ML you get
10iu/1mL: 1 iu per 10 IU (on the syringe – 1/10th the product)
10iu/2mL: 1 iu per 20 IU (on the syringe – still 1/10th the product)

Say you have a 5000iu vial and you add 1ML you get
5000iu/1mL: 500iu per 10 IU
5000iu/2mL: 250iu per 10 IU

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Reconstituting and Measuring bodybuilding Peptides

Reconstituting and Measuring Peptides

Seems this question comes up a lot and have also received PM’s on it so might be worth laying it out in one post as below.

Firstly it’s a good idea to use an alcohol swab to clean the nipples of both your vial of peptide and dilutent before reconstituting.

Most bodybuilding peptides (HGH, MGF, GHRP, HCG) can be reconstituted with sterile water or more commonly with Bacteriostatic Water (BW) which is sterile water with 0.9% Benzyl Alcohol added and maximizes shelf life. BW is readily available and preferred. Some HGH comes with water provided but best to discard this and use BW as generally the supplied water is intended for single use application only.

One important exclusion to the above is IGF. IGF requires reconstituting with 0.6% Acetic Acid (AA) or 10 mM HCl. 0.6% AA provides the maximum shelf life for the product and is preferred.

Before reconstituting we need to determine how much dilutent to add to the bodybuilding peptide. This will vary upon your own preference for measuring the amount for injection of the reconstituted hormone. I will add at the bottom of this post a guide for this purpose.

So we now have our bodybuilding peptide in a lyophilized powder form and our intended dilutent so let’s reconstitute it. Using a regular 3cc pin with 23-25 gauge needle draw up your required dilutent amount and proceed to add it to the vial with your lyophilized powder. This process needs to be done with care as the bodybuilding peptide are relatively unstable. I suggest tilting the vial on a roughly 45 degree angle and very gently adding in the dilutent allowing it to slowly trickle down the side of the vial and then reaching the lyophilized powder at which time it should dissolve into the dilutent. DO NOT squirt your dilutent directly into the vial or onto the lyophilized powder. It might not fully dissolve so again very gently swirl the vial in one direction only until you have a good mix. DO NOT shake the vial or agitate it violently in any way during this process as again this could conceivably degrade your peptide.

Most peptide injections are done using an insulin syringe. Measurements on these are in International Units (iu’s). This is a measurement of the biological effect and applied to your hormone as a standard accepted “Internationally” but don’t get too hung up on that excepting to know it can be applied with surety to your peptides.

So if your using a U100 insulin syringe this means 1iu is 1/100th of a ml or 1ml = 100iu’s. bodybuilding peptides  are generally packaged and labeled in terms of micrograms (mcg’s) which is 1/1000 of a milligram. So 1mg = 1000mcg’s.

Let’s use an example where you have 1mg of peptide which is reasonably common. This then means you have 1000mcgs of that peptide. If you add 1ml of dilutent to that you now have a solution that will provide you with 10mcg’s per IU. If you add 2mls of dilutent your solution becomes 5mcgs per 1iu and so on. Here is a chart for quick reference:

1000mcg/1mL = 10 mcg per IU
1000mcg/2mL = 5.0 mcg per IU
1000mcg/3mL = 3.3 mcg per IU
1000mcg/4mL = 2.5 mcg per IU

Going from above if you have a 2mg vial of lyophilized powder then the equations changes by a multiple of 2 as below:

2000mcg/1mL = 20 mcg per IU
2000mcg/2mL = 10 mcg per IU
2000mcg/3mL = 6.6 mcg per IU
2000mcg/4mL = 5 mcg per IU

A simple reference to refer back to is the following:

Peptide amount in mcgs / dilutent volume in ml/100 = mcg per IU

Should probably add in here with HGH specifically it is often shipped in kits/vials labelled as 5 or 10iu’s per vial rather than in micrograms. The calculations are the same but to expand on this if you have a 10iu vial of HGH and add 1ml of BW to it you now have in solution with 10iu’s per 1ml. We know from our previous calculations that a U100 insulin syringe holds 1ml of liquid. So to measure your HGH in this example divide the total amount of dilutent by the total IU’s of HGH and what we get is 1iu of HGH each 10 tick on the insulin syringe. Chart again below for easier reference:

1ml = 100 IU’s

100 IU (amount of dilutent) / 10 IU (amount of HGH) = 10 for each IU of HGH

If adding 2mls BW:

200 IU / 10 IU = 20

Most bodybuilding peptide injections are done subcutaneously (sub-q) but can also be done intramuscularly (IM). IGF is again the exception here as this peptide should be injected IM otherwise I prefer sub-q for most others.

All bodybuilding peptide should be stored in the refrigerator once reconstituted to ensure maximum shelf life and this does vary. HGH and HCG are typically good for up to 30 days whereas IGF is good for up to 2 years. Keep them away from direct sunlight at all times.

by Access (R.I.P.)

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